| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Original Article |
BACKGROUND: Efficient production of interferons (IFNs) in virally infected cells is an essential aspect of the host defence. The transcription factor complex ISGF3 (IFN-stimulated gene factor 3) was originally identified as a critical mediator of the IFN signal; it is formed upon IFN receptor (IFNR) stimulation and binds to ISREs (IFN-stimulated response elements) to activate IFN-inducible genes. It has recently been shown that the DNA binding component of ISGF3, p48 (ISGF3gamma) also binds to virus-inducible elements in the IFN-alpha/beta genes, suggesting a potential new role of p48 in IFN production. RESULTS: Primary cells from mice with a targeted disruption of the p48 gene show severe defects in virus-induced IFN-alpha/beta gene expression. A similar defect was also observed in cells lacking type I IFNR or Stat1, further demonstrating the role of IFN signalling in the induction of these IFN genes. ISGF3 in fact binds to the virus-inducible elements within the IFN-alpha/beta promoters. We also provide evidence showing that these elements are additionally controlled by an unidentified factor(s) which presumably triggers the primary phase of IFN gene induction. CONCLUSIONS: Our results demonstrate that the IFN signal transducing complex ISGF3 plays a crucial role in IFN production and suggest that ISGF3 may participate directly in the activation of IFN-alpha/beta promoters. This dual function of ISGF3 may insure the efficient operation of this cytokine system in the host defence.
This article has been cited by other articles:
|
|
 |
|
  C. Trumstedt, E. Eriksson, A. M. Lundberg, T.-b. Yang, Z.-q. Yan, H. Wigzell, and M. E. Rottenberg Role of IRAK4 and IRF3 in the control of intracellular infection with Chlamydia pneumoniae J. Leukoc. Biol., June 1, 2007; 81(6): 1591 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ning, L. E. Huye, and J. S. Pagano Regulation of the Transcriptional Activity of the IRF7 Promoter by a Pathway Independent of Interferon Signaling J. Biol. Chem., April 1, 2005; 280(13): 12262 - 12270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rodel, H. Vogelsang, K. Prager, M. Hartmann, K.-H. Schmidt, and E. Straube Role of Interferon-Stimulated Gene Factor 3{gamma} and Beta Interferon in HLA Class I Enhancement in Synovial Fibroblasts upon Infection with Chlamydia trachomatis Infect. Immun., November 1, 2002; 70(11): 6140 - 6146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Remoli, E. Giacomini, G. Lutfalla, E. Dondi, G. Orefici, A. Battistini, G. Uze, S. Pellegrini, and E. M. Coccia Selective Expression of Type I IFN Genes in Human Dendritic Cells Infected with Mycobacterium tuberculosis J. Immunol., July 1, 2002; 169(1): 366 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Barchet, M. Cella, B. Odermatt, C. Asselin-Paturel, M. Colonna, and U. Kalinke Virus-induced Interferon {alpha} Production by a Dendritic Cell Subset in the Absence of Feedback Signaling In Vivo J. Exp. Med., February 19, 2002; 195(4): 507 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Fagerlie, J. Diaz, T. A. Christianson, K. McCartan, W. Keeble, G. R. Faulkner, and G. C. Bagby Functional correction of FA-C cells with FANCC suppresses the expression of interferon {gamma}-inducible genes Blood, May 15, 2001; 97(10): 3017 - 3024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nees, J. M. Geoghegan, T. Hyman, S. Frank, L. Miller, and C. D. Woodworth Papillomavirus Type 16 Oncogenes Downregulate Expression of Interferon-Responsive Genes and Upregulate Proliferation-Associated and NF-{kappa}B-Responsive Genes in Cervical Keratinocytes J. Virol., May 1, 2001; 75(9): 4283 - 4296. [Abstract] [Full Text]
|
|
|
|
|
|
L. Burysek, W.-S. Yeow, B. Lubyová, M. Kellum, S. L. Schafer, Y. Q. Huang, and P. M. Pitha Functional Analysis of Human Herpesvirus 8-Encoded Viral Interferon Regulatory Factor 1 and Its Association with Cellular Interferon Regulatory Factors and p300 J. Virol., September 1, 1999; 73(9): 7334 - 7342. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. TANIGUCHI, N. TANAKA, K. OGASAWARA, S. TAKI, M. SATO, and A. TAKAOKA Transcription Factor IRF-1 and Its Family Members in the Regulation of Host Defense Cold Spring Harb Symp Quant Biol, January 1, 1999; 64(0): 465 - 472. [Abstract] [PDF]
|
|
|
|
 |
|
 Y.-T. Juang, W. Lowther, M. Kellum, W.-C. Au, R. Lin, J. Hiscott, and P. M. Pitha Primary activation of interferon A and interferon B gene transcription by interferon regulatory factor 3 PNAS, August 18, 1998; 95(17): 9837 - 9842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Lin, C. Heylbroeck, P. M. Pitha, and J. Hiscott Virus-Dependent Phosphorylation of the IRF-3 Transcription Factor Regulates Nuclear Translocation, Transactivation Potential, and Proteasome-Mediated Degradation Mol. Cell. Biol., May 1, 1998; 18(5): 2986 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Schafer, R. Lin, P. A. Moore, J. Hiscott, and P. M. Pitha Regulation of Type I Interferon Gene Expression by Interferon Regulatory Factor-3 J. Biol. Chem., January 30, 1998; 273(5): 2714 - 2720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lu, W.-C. Au, W.-S. Yeow, N. Hageman, and P. M. Pitha Regulation of the Promoter Activity of Interferon Regulatory Factor-7 Gene. ACTIVATION BY INTERFERON AND SILENCING BY HYPERMETHYLATION J. Biol. Chem., October 6, 2000; 275(41): 31805 - 31812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Xiao, L. Wang, X. Yang, T. Chen, D. Hodge, P. F. Johnson, and W. Farrar CCAAT/Enhancer-binding Protein beta Mediates Interferon-gamma -induced p48 (ISGF3-gamma ) Gene Transcription in Human Monocytic Cells J. Biol. Chem., June 22, 2001; 276(26): 23275 - 23281. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
