Mutator mutants that show an increased frequency of spontaneous mutation have led to the elucidation of the multiple pathways of spontaneous mutagenesis. 8-Oxo-dGTP (8-oxo-7,8-dihydrodeoxyguanosine triphosphate) is formed in the nucleotide pool of a cell during normal cellular metabolism, and when it is incorporated into DNA causes mutation. MutT protein of Escherichia coli and related mammalian enzymes specifically degrade 8-oxo-dGTP to 8-oxo-dGMP, thereby preventing occurrence of transversion mutation. The gene encoding the human enzyme, designated MTH1 (for mutT homologue 1), maps to chromosome 7p22. These proteins may be responsible for genomic stability.

This article has been cited by other articles:

				Y. Sakai, M. Furuichi, M. Takahashi, M. Mishima, S. Iwai, M. Shirakawa, and Y. Nakabeppu A Molecular Basis for the Selective Recognition of 2-Hydroxy-dATP and 8-Oxo-dGTP by Human MTH1 J. Biol. Chem., March 1, 2002; 277(10): 8579 - 8587. [Abstract] [Full Text] [PDF]

				T. Kai, R. Matsunaga, M. Eguchi, H. Kamiya, H. Kasai, M. Suzuki, and S. Izuta An oxidized nucleotide affects DNA replication through activation of protein kinases in Xenopus egg lysates Nucleic Acids Res., January 15, 2002; 30(2): 569 - 573. [Abstract] [Full Text] [PDF]

				T. Tsuzuki, A. Egashira, H. Igarashi, T. Iwakuma, Y. Nakatsuru, Y. Tominaga, H. Kawate, K. Nakao, K. Nakamura, F. Ide, et al. Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase PNAS, September 25, 2001; 98(20): 11456 - 11461. [Abstract] [Full Text] [PDF]

				T. Iida, A. Furuta, M. Kawashima, J.-i. Nishida, Y. Nakabeppu, and T. Iwaki Accumulation of 8-oxo-2'-deoxyguanosine and increased expression of hMTH1 protein in brain tumors Neuro-oncol, April 1, 2001; 3(2): 73 - 81. [Abstract] [PDF]

				S. Breinig, E. Schiltz, and G. Fuchs Genes Involved in Anaerobic Metabolism of Phenol in the Bacterium Thauera aromatica J. Bacteriol., October 15, 2000; 182(20): 5849 - 5863. [Abstract] [Full Text]

				H. Shimokawa, Y. Fujii, M. Furuichi, M. Sekiguchi, and Y. Nakabeppu Functional significance of conserved residues in the phosphohydrolase module of Escherichia coli MutT protein Nucleic Acids Res., September 1, 2000; 28(17): 3240 - 3249. [Abstract] [Full Text] [PDF]

				K. Miyako, C. Takamatsu, S. Umeda, T. Tajiri, M. Furuichi, Y. Nakabeppu, M. Sekiguchi, N. Hamasaki, K. Takeshige, and D. Kang Accumulation of Adenine DNA Glycosylase-sensitive Sites in Human Mitochondrial DNA J. Biol. Chem., April 14, 2000; 275(16): 12326 - 12330. [Abstract] [Full Text] [PDF]

				T. Ohtsubo, K. Nishioka, Y. Imaiso, S. Iwai, H. Shimokawa, H. Oda, T. Fujiwara, and Y. Nakabeppu Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria Nucleic Acids Res., March 15, 2000; 28(6): 1355 - 1364. [Abstract] [Full Text] [PDF]

				Y. Fujii, H. Shimokawa, M. Sekiguchi, and Y. Nakabeppu Functional Significance of the Conserved Residues for the 23-Residue Module among MTH1 and MutT Family Proteins J. Biol. Chem., December 31, 1999; 274(53): 38251 - 38259. [Abstract] [Full Text] [PDF]

				K. Nishioka, T. Ohtsubo, H. Oda, T. Fujiwara, D. Kang, K. Sugimachi, and Y. Nakabeppu Expression and Differential Intracellular Localization of Two Major Forms of Human 8-Oxoguanine DNA Glycosylase Encoded by Alternatively Spliced OGG1 mRNAs Mol. Biol. Cell, May 1, 1999; 10(5): 1637 - 1652. [Abstract] [Full Text]

				H. Oda, Y. Nakabeppu, M. Furuichi, and M. Sekiguchi Regulation of Expression of the Human MTH1 Gene Encoding 8-Oxo-dGTPase. ALTERNATIVE SPLICING OF TRANSCRIPTION PRODUCTS J. Biol. Chem., July 11, 1997; 272(28): 17843 - 17850. [Abstract] [Full Text] [PDF]

				J. L. Cartwright, L. Gasmi, D. G. Spiller, and A. G. McLennan The Saccharomyces cerevisiae PCD1 Gene Encodes a Peroxisomal Nudix Hydrolase Active toward Coenzyme A and Its Derivatives J. Biol. Chem., October 13, 2000; 275(42): 32925 - 32930. [Abstract] [Full Text] [PDF]