| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Biology, School of Sciences, Kyushu University, Fukuoka 812-8581, Japan
2 Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan
3 Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980-8578, Japan
Human WRNIP1, a Werner DNA helicase interacting protein 1, was expressed in insect cells and E. coli. The purified protein behaved as a homo-oligomeric complex with a native molecular mass indicative of an octamer, and the complex copurified with an ATPase activity that was stimulated by double-stranded DNA ends. As suggested by genetic studies of budding yeast WRNIP1/Mgs1, the purified human WRNIP1 complex interacted physically with human DNA polymerase 
(pol ), stimulating its DNA synthesis activity more than fivefold in the presence or absence of proliferating cell nuclear antigen. Analysis of reaction products demonstrated the stimulation to be partly due to an increased processivity of pol but more importantly to an increase in its initiation frequency. Addition of ATP to reactions partially suppressed stimulation by WRNIP1. Furthermore, a mutant WRNIP1 lacking ATPase activity could stimulate pol normally but was insensitive to suppression by ATP. These results indicate that WRNIP1 functions as a modulator for initiation or restart events during pol -mediated DNA synthesis and that its ATPase activity is utilized to sense DNA ends and to regulate the extent of stimulation.
aPresent address: Amersham Biosciences K.K. Sanken Bldg. 3-25-1 Hyakunincho, Shinjuku-ku, Tokyo 169–0073, Japan * Correspondence: E-mail: ttsurscb{at}mbox.nc.kyushu-u.ac.jp
This article has been cited by other articles:
|
|
 |
|
  R. A. Bish and M. P. Myers Werner Helicase-interacting Protein 1 Binds Polyubiquitin via Its Zinc Finger Domain J. Biol. Chem., August 10, 2007; 282(32): 23184 - 23193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhang, Y. Zhou, S. Trusa, X. Meng, E. Y. C. Lee, and M. Y. W. T. Lee A Novel DNA Damage Response: RAPID DEGRADATION OF THE p12 SUBUNIT OF DNA POLYMERASE {delta} J. Biol. Chem., May 25, 2007; 282(21): 15330 - 15340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hishida, T. Ohya, Y. Kubota, Y. Kamada, and H. Shinagawa Functional and Physical Interaction of Yeast Mgs1 with PCNA: Impact on RAD6-Dependent DNA Damage Tolerance. Mol. Cell. Biol., July 1, 2006; 26(14): 5509 - 5517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sasakawa, T. Fukui, and S. Waga Accumulation of FFA-1, the Xenopus Homolog of Werner Helicase, and DNA Polymerase {delta} on Chromatin in Response to Replication Fork Arrest J. Biochem., July 1, 2006; 140(1): 95 - 103. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Kim, Y.-H. Kang, H.-J. Kang, D.-H. Kim, G.-H. Ryu, M.-J. Kang, and Y.-S. Seo In vivo and in vitro studies of Mgs1 suggest a link between genome instability and Okazaki fragment processing Nucleic Acids Res., October 26, 2005; 33(19): 6137 - 6150. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
