HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Urahama, N. Articles by Matsui, T. Search for Related Content PubMed PubMed Citation Articles by Urahama, N. Articles by Matsui, T.

¹ Division of Hematology/Oncology, Department of Medicine, Kobe University School of Medicine,Kobe 650-0017, Japan
² Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan
³ Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021-6399, USA

The TRAP220 subunit of the thyroid hormone receptor-associated polypeptide transcription coactivator complex (TRAP/Mediator complex), mammalian counterpart of the yeast Mediator complex, is proposed to act on a variety of major and specific biological events through physical interactions with nuclear receptors. The vitamin D receptor (VDR) and retinoic acid receptor (RAR), coupled with retinoid X receptor (RXR), are nuclear receptors which have important roles for monopoiesis and granulopoiesis, respectively. In this study, we present the functional role of TRAP220 in nuclear receptor-mediated monopoiesis and granulopoiesis. The mouse Trap220^–/– yolk sac hematopoietic progenitor cells were resistant to 1,25-dihydroxyvitamin D₃-stimulated differentiation into monocytes/macrophages. Furthermore, flow cytometric analyses showed that HL-60 cells, human promyelocytic leukemia cell line, wherein TRAP220 was down-regulated, did not differentiate efficiently into monocytes and granulocytes by stimulation with 1,25-dihydroxyvitamin D₃ and all-trans retinoic acid, correspondingly. The expression of direct target genes of VDR or RAR, as well as the differentiation marker genes, was low in the knockdown cells. These results indicated a crucial role of TRAP220 in the optimal VDR- and RAR-mediated myelomonocytic differentiation processes in mammalian hematopoiesis.

^* Correspondence: E-mail: itomi{at}med.kobe-u.ac.jp

This article has been cited by other articles:

				J. Moses, A. Goodchild, and L. P. Rivory Intended transcriptional silencing with siRNA results in gene repression through sequence-specific off-targeting RNA, February 1, 2010; 16(2): 430 - 441. [Abstract] [Full Text] [PDF]

				Y. Nakamura, Y. Xing, H. Sasano, and W. E. Rainey The Mediator Complex Subunit 1 Enhances Transcription of Genes Needed for Adrenal Androgen Production Endocrinology, September 1, 2009; 150(9): 4145 - 4153. [Abstract] [Full Text] [PDF]

				S. Garapaty, M. A. Mahajan, and H. H. Samuels Components of the CCR4-NOT Complex Function as Nuclear Hormone Receptor Coactivators via Association with the NRC-interacting Factor NIF-1 J. Biol. Chem., March 14, 2008; 283(11): 6806 - 6816. [Abstract] [Full Text] [PDF]

				M. Stumpf, C. Waskow, M. Krotschel, D. van Essen, P. Rodriguez, X. Zhang, B. Guyot, R. G. Roeder, and T. Borggrefe The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220 PNAS, December 5, 2006; 103(49): 18504 - 18509. [Abstract] [Full Text] [PDF]