| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Graduate Program in Biophysics and Biochemistry, School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
2 Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan
The Rho GTPase acts as a binary molecular switch by converting between a GDP-bound inactive and a GTP-bound active conformational state. The guanine nucleotide exchange factors (GEFs) are critical activators of Rho. Rho1 has been shown to regulate actin cytoskeleton and cell wall synthesis in the fission yeast Schizosaccharomyces pombe. Here we studied function of fission yeast RhoGEFs, Rgf1, Rgf2, and Rgf3. It was shown that these proteins have similar molecular structures, and function as GEFs for Rho1. Disruption of either rgf1 or rgf2 did not show a serious effect on the cell. On the other hand, disruption of rgf3 caused severe defects in contractile ring formation, F-actin patch localization, and septation during cytokinesis. Rgf1 and Rgf2 were localized to the cell ends during interphase and the septum. Rgf3 formed a ring at the division site, which was located outside the contractile ring and inside the septum where Rho1 was accumulated. In summary, Rgf1 and Rgf2 show functional redundancy, and roles of these RhoGEFs are likely to be different from that of Rgf3. Rho1 is likely to be activated by Rgf3 at the division site, and involved in contractile ring formation and/or maintenance and septation.
aPresent address: Institute of Biological Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8577, Japan. * Correspondence: E-mail: mabuchi{at}ims.u-tokyo.ac.jp
This article has been cited by other articles:
|
|
 |
|
  P. Garcia, I. Garcia, F. Marcos, G. R. de Garibay, and Y. Sanchez Fission Yeast Rgf2p Is a Rho1p Guanine Nucleotide Exchange Factor Required for Spore Wall Maturation and for the Maintenance of Cell Integrity in the Absence of Rgf1p Genetics, April 1, 2009; 181(4): 1321 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Garcia, V. Tajadura, and Y. Sanchez The Rho1p Exchange Factor Rgf1p Signals Upstream from the Pmk1 Mitogen-activated Protein Kinase Pathway in Fission Yeast Mol. Biol. Cell, January 1, 2009; 20(2): 721 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pinar, P. M. Coll, S. A. Rincon, and P. Perez Schizosaccharomyces pombe Pxl1 Is a Paxillin Homologue That Modulates Rho1 Activity and Participates in Cytokinesis Mol. Biol. Cell, April 1, 2008; 19(4): 1727 - 1738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yoshida, K. Kono, D. M. Lowery, S. Bartolini, M. B. Yaffe, Y. Ohya, and D. Pellman Polo-Like Kinase Cdc5 Controls the Local Activation of Rho1 to Promote Cytokinesis Science, July 7, 2006; 313(5783): 108 - 111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q.-W. Jin, M. Zhou, A. Bimbo, M. K. Balasubramanian, and D. McCollum A Role for the Septation Initiation Network in Septum Assembly Revealed by Genetic Analysis of sid2-250 Suppressors Genetics, April 1, 2006; 172(4): 2101 - 2112. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
