HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tamaki, H. Articles by Kumagai, H. Search for Related Content PubMed PubMed Citation Articles by Tamaki, H. Articles by Kumagai, H.

¹ Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan
² Institute for Advanced Technology, Suntory Research Center, Osaka 618-8503, Japan
³ Division of Gene Research Center for Biological Resources and Informatics, Tokyo Institute of Technology, Yokohama 226-8501, Japan
⁴ School of Life Sciences and Biotechnology, Korea University, Anam-Dong, Sungbuk-ku, 136-701 Seoul, Korea

In the yeast, Saccharomyces cerevisiae, cell size is affected by the kind of carbon source in the medium. Here, we present evidence that the Gpr1 receptor and Gpa2 G subunit are required for both maintenance and modulation of cell size in response to glucose. In the presence of glucose, mutants lacking GPR1 or GPA2 gene showed smaller cells than the wild-type strain. Physiological studies revealed that protein synthesis rate was reduced in the mutant strains indicating that reduced growth rate, while the level of mRNAs for CLN1, 2 and 3 was not affected in all strains. Gene chip analysis also revealed a down-regulation in the expression of genes related to biosynthesis of not only protein but also other cellular component in the mutant strains. We also show that GPR1 and GPA2 are required for a rapid increase in cell size in response to glucose. Wild-type cells grown in ethanol quickly increased in size by addition of glucose, while little change was observed in the mutant strains, in which glucose-dependent cell cycle arrest caused by CLN1 repression was somewhat alleviated. Our study indicates that the yeast G-protein coupled receptor system consisting of Gpr1 and Gpa2 regulates cell size by affecting both growth rate and cell division.

^* Correspondence: E-mail: noritama{at}kais.kyoto-u.ac.jp

This article has been cited by other articles:

				P. J. Doyle, H. Saeed, A. Hermans, S. C. Gleddie, G. Hussack, M. Arbabi-Ghahroudi, C. Seguin, M. E. Savard, C. R. MacKenzie, and J. C. Hall Intracellular Expression of a Single Domain Antibody Reduces Cytotoxicity of 15-Acetyldeoxynivalenol in Yeast J. Biol. Chem., December 11, 2009; 284(50): 35029 - 35039. [Abstract] [Full Text] [PDF]

				T. Niranjan, X. Guo, J. Victor, A. Lu, and J. P. Hirsch Kelch Repeat Protein Interacts with the Yeast G{alpha} Subunit Gpa2p at a Site That Couples Receptor Binding to Guanine Nucleotide Exchange J. Biol. Chem., August 17, 2007; 282(33): 24231 - 24238. [Abstract] [Full Text] [PDF]

				H. X. Wang, R. R. Weerasinghe, T. D. Perdue, N. G. Cakmakci, J. P. Taylor, W. F. Marzluff, and A. M. Jones A Golgi-localized Hexose Transporter Is Involved in Heterotrimeric G Protein-mediated Early Development in Arabidopsis Mol. Biol. Cell, October 1, 2006; 17(10): 4257 - 4269. [Abstract] [Full Text] [PDF]

				M. C. Overton, S. L. Chinault, and K. J. Blumer Oligomerization of G-Protein-Coupled Receptors: Lessons from the Yeast Saccharomyces cerevisiae Eukaryot. Cell, December 1, 2005; 4(12): 1963 - 1970. [Full Text] [PDF]