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¹ Division of Molecular and Cell Biology, Institute for Medical Science
² Department of Gastroenterology, Dokkyo University School of Medicine, 880 Kitakobayashi, Mibu-machi, Tochigi 321-0293, Japan

-Taxilin is a novel binding partner of the syntaxin family, which is implicated in intracellular vesicle traffic. We have here found that -taxilin interacts with the nascent polypeptide-associated complex (NAC), which is involved in transferring growing nascent polypeptide chains to appropriate co-translationally acting factors. NAC is composed of two subunits, - and ßNACs. Both these subunits bound to -taxilin through its C-terminal coiled-coil region in dose-dependent and saturable manners. The interactions of -taxilin with NAC and NAC but not with ßNAC were inhibited by syntaxin-4, indicating that -taxilin binds to NAC mainly through its interaction with NAC. When NAC was over-expressed in COS-7 cells, NAC was distributed in the cytosol and nucleus. However, co-expression of the -taxilin fragment containing the NAC-binding region eliminated the nuclear distribution of over-expressed NAC. Moreover, other taxilin family members, ß- and -taxilins, also bound to NAC and thereby affected the nuclear distribution of over-expressed NAC. Taken together with the evidence that NAC functions in the nucleus as a transcriptional coactivator, our results raise the possibility that the taxilin family is involved not only in the translational process through its interaction with NAC but also in the transcriptional process through its interaction with NAC alone.

^* Correspondence: E-mail: hiro-sh{at}dokkyomed.ac.jp

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