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Kazuaki Tokunaga^1,, Toshiharu Shibuya^2,a,, Yo Ishihama³, Hisashi Tadakuma³, Miyuki Ide¹, Minoru Yoshida⁴, Takashi Funatsu^3,5, Yasumi Ohshima² and Tokio Tani^1,*

¹ Department of Biological Science, Faculty of Science, Kumamoto University, Kumamoto 860-8555, Japan
² Department of Biology, Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, Japan
³ Department of Physics, School of Science and Engineering, Waseda University, Okubo, Tokyo 169-8555, Japan
⁴ Chemical Genetics Laboratory, RIKEN, Wako, Saitama 351-0198, Japan
⁵ Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan

In eukaryotic cells, export of mRNA from the nucleus to the cytoplasm is one of the essential steps in gene expression. To examine mechanisms involved in the nucleocytoplasmic transport of mRNA, we microinjected fluorescently labeled fushi tarazu (ftz) pre-mRNA into the nuclei of HeLa cells. The injected intron-containing ftz pre-mRNA was distributed to the SC35 speckles and exported to the cytoplasm after splicing by an energy-requiring active process. In contrast, the injected intron-less ftz mRNA was diffusely distributed in the nucleus and then presumably degraded. Interestingly, export of the ftz pre-mRNA was inhibited by treatment with transcriptional inhibitors (actinomycin D, -amanitin or DRB). Cells treated with transcriptional inhibitor showed foci enriched with the injected mRNA, which localize side by side with SC35 speckles. Those nuclear foci, referred to as TIDRs (transcriptional-inactivation dependent RNA domain), do not overlap with paraspeckles. In addition, in situ hybridization analysis revealed that the export of endogenous poly(A)⁺ mRNA is also affected by transcriptional inactivation. These results suggest that nuclear mRNA export is coupled to ongoing gene transcription in mammalian cells.

^aPresent address: Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA.

These authors contributed equally to this work.

^* Correspondence: E-mail: ttani{at}sci.kumamoto-u.ac.jp

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