| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 The G0 Cell Unit, Okinawa Institute of Science and Technology Promotion Corporation, Suzaki 12-22, Uruma, Okinawa 904-2234, Japan
2 CREST Research Program, Japan Science and Technology Corporation, and
3 The Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
Nutrients are essential for cell growth and division. Screening of Schizosaccharomyces pombe temperature-sensitive strains led to the isolation of a nutrient-insensitive mutant, tor2-287. This mutant produces a nitrogen starvation-induced arrest phenotype in rich media, fails to recover from the arrest, and is hypersensitive to rapamycin. The L2048S substitution mutation in the catalytic domain in close proximity to the adenine base of ATP is unique as it is the sole known genetic cause of rapamycin hypersensitivity. Localization of Tor2 was speckled in the vegetative cytoplasm, and both speckled and membranous in the arrested cell cytoplasm. Using mass spectroscopic analysis, we identified six subunits (Tco89, Bit61, Toc1, Tel2, Tti1 and Cka1) that, in addition to the six previously identified subunits (Tor1, Tor2, Mip1/Raptor, Ste20/Rictor, Sin1/Avo1 and Wat1/Lst8), comprise the TOR complexes (TORCs). All of the subunits so far examined are multiply phosphorylated. Tel2 bound to Tti1 interacts with various phosphatidyl inositol kinase (PIK)-related kinases including Tra1, Tra2 and Rad3, as well as Tor1 and Tor2. Schizosaccharomyces pombe TORCs should thus be functionally redundant and might be broadly regulated through different subunits that are either common or specific to the two TORCs, or even common to various PIK-related kinases. Functional redundancy of the TORCs may explain the rapamycin hypersensitivity of tor2-287.
aThese authors have contributed equally to this article. * Correspondence: E-mail: nagao{at}oist.jp or yanagida{at}kozo.lif.kyoto-u.ac.jp
This article has been cited by other articles:
|
|
 |
|
  K. Shiozaki Nutrition-Minded Cell Cycle Sci. Signal., November 10, 2009; 2(96): pe74 - pe74. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. C. Dibble, J. M. Asara, and B. D. Manning Characterization of Rictor Phosphorylation Sites Reveals Direct Regulation of mTOR Complex 2 by S6K1 Mol. Cell. Biol., November 1, 2009; 29(21): 5657 - 5670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Murai, Y. Nakase, K. Fukuda, Y. Chikashige, C. Tsutsumi, Y. Hiraoka, and T. Matsumoto Distinctive Responses to Nitrogen Starvation in the Dominant Active Mutants of the Fission Yeast Rheb GTPase Genetics, October 1, 2009; 183(2): 517 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schonbrun, D. Laor, L. Lopez-Maury, J. Bahler, M. Kupiec, and R. Weisman TOR Complex 2 Controls Gene Silencing, Telomere Length Maintenance, and Survival under DNA-Damaging Conditions Mol. Cell. Biol., August 15, 2009; 29(16): 4584 - 4594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hartmuth and J. Petersen Fission yeast Tor1 functions as part of TORC1 to control mitotic entry through the stress MAPK pathway following nutrient stress J. Cell Sci., June 1, 2009; 122(11): 1737 - 1746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hanyu, K. K. Imai, Y. Kawasaki, T. Nakamura, Y. Nakaseko, K. Nagao, A. Kokubu, M. Ebe, A. Fujisawa, T. Hayashi, et al. Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor Genes Cells, May 1, 2009; 14(5): 539 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. R. Danielsen, D. H. Larsen, K. B. Schou, R. Freire, J. Falck, J. Bartek, and J. Lukas HCLK2 Is Required for Activity of the DNA Damage Response Kinase ATR J. Biol. Chem., February 13, 2009; 284(7): 4140 - 4147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Helmlinger, S. Marguerat, J. Villen, S. P. Gygi, J. Bahler, and F. Winston The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8 Genes & Dev., November 15, 2008; 22(22): 3184 - 3195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Anderson, D. Korkin, D. L. Smith, S. Makovets, J. J. Seidel, A. Sali, and E. H. Blackburn Tel2 mediates activation and localization of ATM/Tel1 kinase to a double-strand break Genes & Dev., April 1, 2008; 22(7): 854 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kanoh and M. Yanagida Tel2: a common partner of PIK-related kinases and a link between DNA checkpoint and nutritional response? Genes Cells, December 1, 2007; 12(12): 1301 - 1304. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
