| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Cell Biology, Division of Molecular Cell Biology, National Institute for Basic Biology, Nishigonaka 38, Myodaiji-cho, Okazaki, 444-8585 Japan
2 Department of Basic Biology, The Graduate University for Advanced Studies, Nishigonaka 38, Myodaiji-cho, Okazaki, 444-8585 Japan
Autophagy is a bulk degradation process that is conserved in eukaryotic cells and functions in the turnover of cytoplasmic materials and organelles. When eukaryotic cells face nutrient starvation, the autophagosome, a double-membraned organelle, is generated from the pre-autophagosomal structure (PAS). In the yeast Saccharomyces cerevisiae, 16 ATG (autophagy-related) genes are essential for autophagosome formation. Most of the Atg proteins are involved in the PAS, leading to autophagosome production. However, the mechanism of PAS organization remains to be elucidated. Here, we performed a systematic and quantitative analysis by fluorescence microscopy to develop a hierarchy map of Atg proteins involved in PAS organization. This analysis suggests that Atg17p is the most basic protein in PAS organization: when it is specifically targeted to the plasma membrane, other Atg proteins are recruited to that location, suggesting that Atg17p acts as a scaffold protein to organize Atg proteins to the PAS.
* Correspondence: E-mail: yohsumi{at}nibb.ac.jp
This article has been cited by other articles:
|
|
 |
|
  Y. Kamada, K.-i. Yoshino, C. Kondo, T. Kawamata, N. Oshiro, K. Yonezawa, and Y. Ohsumi Tor Directly Controls the Atg1 Kinase Complex To Regulate Autophagy Mol. Cell. Biol., February 15, 2010; 30(4): 1049 - 1058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-L. Yen, T. Shintani, U. Nair, Y. Cao, B. C. Richardson, Z. Li, F. M. Hughson, M. Baba, and D. J. Klionsky The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy J. Cell Biol., January 11, 2010; 188(1): 101 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Stephan, Y.-Y. Yeh, V. Ramachandran, S. J. Deminoff, and P. K. Herman The Tor and PKA signaling pathways independently target the Atg1/Atg13 protein kinase complex to control autophagy PNAS, October 6, 2009; 106(40): 17049 - 17054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Y. Nazarko, J.-C. Farre, and S. Subramani Peroxisome Size Provides Insights into the Function of Autophagy-related Proteins Mol. Biol. Cell, September 1, 2009; 20(17): 3828 - 3839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sekito, T. Kawamata, R. Ichikawa, K. Suzuki, and Y. Ohsumi Atg17 recruits Atg9 to organize the pre-autophagosomal structure Genes Cells, May 1, 2009; 14(5): 525 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bommareddy, E.-R. Hahm, D. Xiao, A. A. Powolny, A. L. Fisher, Y. Jiang, and S. V. Singh Atg5 Regulates Phenethyl Isothiocyanate-Induced Autophagic and Apoptotic Cell Death in Human Prostate Cancer Cells Cancer Res., April 15, 2009; 69(8): 3704 - 3712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hosokawa, T. Hara, T. Kaizuka, C. Kishi, A. Takamura, Y. Miura, S.-i. Iemura, T. Natsume, K. Takehana, N. Yamada, et al. Nutrient-dependent mTORC1 Association with the ULK1-Atg13-FIP200 Complex Required for Autophagy Mol. Biol. Cell, April 1, 2009; 20(7): 1981 - 1991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. H. Jung, C. B. Jun, S.-H. Ro, Y.-M. Kim, N. M. Otto, J. Cao, M. Kundu, and D.-H. Kim ULK-Atg13-FIP200 Complexes Mediate mTOR Signaling to the Autophagy Machinery Mol. Biol. Cell, April 1, 2009; 20(7): 1992 - 2003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Sun, W. Fan, K. Chen, X. Ding, S. Chen, and Q. Zhong Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase PNAS, December 9, 2008; 105(49): 19211 - 19216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. He, M. Baba, Y. Cao, and D. J. Klionsky Self-Interaction Is Critical for Atg9 Transport and Function at the Phagophore Assembly Site during Autophagy Mol. Biol. Cell, December 1, 2008; 19(12): 5506 - 5516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Itakura, C. Kishi, K. Inoue, and N. Mizushima Beclin 1 Forms Two Distinct Phosphatidylinositol 3-Kinase Complexes with Mammalian Atg14 and UVRAG Mol. Biol. Cell, December 1, 2008; 19(12): 5360 - 5372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Geng, M. Baba, U. Nair, and D. J. Klionsky Quantitative analysis of autophagy-related protein stoichiometry by fluorescence microscopy J. Cell Biol., October 23, 2008; 182(1): 129 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Juhasz, J. H. Hill, Y. Yan, M. Sass, E. H. Baehrecke, J. M. Backer, and T. P. Neufeld The class III PI(3)K Vps34 promotes autophagy and endocytosis but not TOR signaling in Drosophila J. Cell Biol., October 17, 2008; 181(4): 655 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hara, A. Takamura, C. Kishi, S.-i. Iemura, T. Natsume, J.-L. Guan, and N. Mizushima FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells J. Cell Biol., October 14, 2008; 181(3): 497 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Krick, Y. Muehe, T. Prick, S. Bremer, P. Schlotterhose, E.-L. Eskelinen, J. Millen, D. S. Goldfarb, and M. Thumm Piecemeal Microautophagy of the Nucleus Requires the Core Macroautophagy Genes Mol. Biol. Cell, October 1, 2008; 19(10): 4492 - 4505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Obara, T. Sekito, K. Niimi, and Y. Ohsumi The Atg18-Atg2 Complex Is Recruited to Autophagic Membranes via Phosphatidylinositol 3-Phosphate and Exerts an Essential Function J. Biol. Chem., August 29, 2008; 283(35): 23972 - 23980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cao, H. Cheong, H. Song, and D. J. Klionsky In vivo reconstitution of autophagy in Saccharomyces cerevisiae J. Cell Biol., August 26, 2008; 182(4): 703 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Axe, S. A. Walker, M. Manifava, P. Chandra, H. L. Roderick, A. Habermann, G. Griffiths, and N. T. Ktistakis Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum J. Cell Biol., August 26, 2008; 182(4): 685 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Oh-oka, H. Nakatogawa, and Y. Ohsumi Physiological pH and Acidic Phospholipids Contribute to Substrate Specificity in Lipidation of Atg8 J. Biol. Chem., August 8, 2008; 283(32): 21847 - 21852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Xie, U. Nair, and D. J. Klionsky Atg8 Controls Phagophore Expansion during Autophagosome Formation Mol. Biol. Cell, August 1, 2008; 19(8): 3290 - 3298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Obara, T. Noda, K. Niimi, and Y. Ohsumi Transport of phosphatidylinositol 3-phosphate into the vacuole via autophagic membranes in Saccharomyces cerevisiae. Genes Cells, June 1, 2008; 13(6): 537 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kawamata, Y. Kamada, Y. Kabeya, T. Sekito, and Y. Ohsumi Organization of the Pre-autophagosomal Structure Responsible for Autophagosome Formation Mol. Biol. Cell, May 1, 2008; 19(5): 2039 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujita, T. Itoh, H. Omori, M. Fukuda, T. Noda, and T. Yoshimori The Atg16L Complex Specifies the Site of LC3 Lipidation for Membrane Biogenesis in Autophagy Mol. Biol. Cell, May 1, 2008; 19(5): 2092 - 2100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Phillips, A. Suttangkakul, and R. D. Vierstra The ATG12-Conjugating Enzyme ATG10 Is Essential for Autophagic Vesicle Formation in Arabidopsis thaliana Genetics, March 1, 2008; 178(3): 1339 - 1353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cheong, U. Nair, J. Geng, and D. J. Klionsky The Atg1 Kinase Complex Is Involved in the Regulation of Protein Recruitment to Initiate Sequestering Vesicle Formation for Nonspecific Autophagy in Saccharomyces cerevisiae Mol. Biol. Cell, February 1, 2008; 19(2): 668 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Picazarri, K. Nakada-Tsukui, and T. Nozaki Autophagy during Proliferation and Encystation in the Protozoan Parasite Entamoeba invadens Infect. Immun., January 1, 2008; 76(1): 278 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hanada, N. N. Noda, Y. Satomi, Y. Ichimura, Y. Fujioka, T. Takao, F. Inagaki, and Y. Ohsumi The Atg12-Atg5 Conjugate Has a Novel E3-like Activity for Protein Lipidation in Autophagy J. Biol. Chem., December 28, 2007; 282(52): 37298 - 37302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mizushima Autophagy: process and function Genes & Dev., November 15, 2007; 21(22): 2861 - 2873. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
