GTC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Genes to Cells (2007) 12, 511-519. doi:10.1111/j.1365-2443.2007.01070.x
© 2007 Blackwell Publishing or its licensors
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsuboi, T.
Right arrow Articles by Fukuda, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Tsuboi, T.
Right arrow Articles by Fukuda, M.

Synaptotagmin VII modulates the kinetics of dense-core vesicle exocytosis in PC12 cells

 Takashi Tsuboi1 and Mitsunori Fukuda1,2,*

1 Fukuda Initiative Research Unit, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
2 Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan

In our previous study, we showed that PC12 cell lines stably expressing synaptotagmin (Syt) VII have greater ability to release hormones Ca2+-dependently than the original PC12 cells. However, the precise molecular mechanism of the enhancement of hormone secretion by Syt VII has never been elucidated. In this study, we established a PC12 cell line that stably expresses Syt VII-green fluorescent protein (Syt VII-GFP) or its Ca2+-binding-site-deficient mutant (D172N/D303N substitutions; Syt VII-DN-GFP), and examined the effect of Syt VII-GFP expression on the kinetics of dense-core vesicle exocytosis by total internal reflection fluorescence (TIRF) microscopy. Both Syt VII-GFP and Syt VII-DN-GFP co-localized well with dense-core vesicle markers, monomeric red fluorescent protein (mRFP)-tagged neuropeptide Y (NPY-mRFP) and cyan fluorescent protein (CFP)-tagged tissue plasminogen activator (tPA-CFP). Expression of Syt VII-GFP enhanced the number of dense-core vesicle exocytotic events, whereas expression of Syt VII-DN-GFP or knockdown of Syt VII-GFP with specific small interfering RNA (siRNA) attenuated the number of exocytotic events. Monitoring individual tPA-CFP release events revealed that "full release" events are increased in Syt VII-GFP-expressing cells, but not in Syt VII-DN-GFP-expressing or Syt VII-silenced cells. Our data indicate that Syt VII modulates the kinetics of Ca2+-dependent dense-core vesicle exocytosis in neuroendocrine PC12 cells, possibly by modulating fusion pore opening.

Communicated by: Kozo Kaibuchi

* Correspondence: E-mail: nori{at}mail.tains.tohoku.ac.jp




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J.-S. Schonn, A. Maximov, Y. Lao, T. C. Sudhof, and J. B. Sorensen
Synaptotagmin-1 and -7 are functionally overlapping Ca2+ sensors for exocytosis in adrenal chromaffin cells
PNAS, March 11, 2008; 105(10): 3998 - 4003.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L. Osborne, P. J. Wen, C. Boucheron, H. N. Nguyen, M. Hayakawa, H. Kaizawa, P. J. Parker, N. Vitale, and F. A. Meunier
PIKfyve Negatively Regulates Exocytosis in Neurosecretory Cells
J. Biol. Chem., February 1, 2008; 283(5): 2804 - 2813.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
B. R. Gauthier, D. L. Duhamel, M. Iezzi, S. Theander, F. Saltel, M. Fukuda, B. Wehrle-Haller, and C. B. Wollheim
Synaptotagmin VII splice variants {alpha}, , and {delta} are expressed in pancreatic -cells and regulate insulin exocytosis
FASEB J, January 1, 2008; 22(1): 194 - 206.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
Copyright © 2007 by Wiley-Blackwell Publishing.