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¹ Biochemistry Group, Leibniz Institute for Age Research–Fritz-Lipmann-Institute e. V., Beutenbergstraße 11, D-07745 Jena, Germany
² GSF, Institute of Immunology, Marchioninistr. 25, D-81377 München, Germany
³ National University of Ireland Galway, Department of Biochemistry, Galway, Ireland

Cdc45 is an essential cellular protein that functions in both the initiation and elongation of DNA replication. Here, we analyzed the localization of human Cdc45 and its interactions with other proteins during the cell cycle. Human Cdc45 showed a diffuse distribution in G1 phase, a spot-like pattern in S and G2, and again a diffuse distribution in M phase of the cell cycle. The co-localization of Cdc45 with active replication sites during S phase suggested that the human Cdc45 protein was part of the elongation complex. This view was corroborated by findings that Cdc45 interacted with the elongating DNA polymerases and , with Psf2, which is a component of the GINS complex as well as with Mcm5 and 7, subunits of the putative replicative DNA helicase complex. Hence, Cdc45 may play an important role in elongation of DNA replication by bridging the processive DNA polymerases and with the replicative helicase in the elongating machinery.

^a Present address: Radiation Oncology and Biology, Radiobiology Research Institute, Churchill Hospital Headington, Oxford OX3 7LJ, UK.

^* Correspondence: E-mail: fgrosse{at}fli-leibniz.de or h.nasheuer{at}nuigalway.ie