HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kurihara, A. Articles by Ikawa, S. Search for Related Content PubMed Articles by Kurihara, A. Articles by Ikawa, S.

¹ Center for Interdisciplinary Research, Tohoku University, Aramaki, 6-3 Aoba, Aoba-ku, Sendai, Japan 980-8578
² Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai, Japan 980-8575
³ Laboratory of Cell Differentiation, Graduate School of Life Sciences, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai, Japan 980-8575
⁴ Department of Dermatology, Tohoku University, Graduate School of Medicine, 2-1 Seiryo-mach, Aoba-ku, Sendai, Japan 980-8574

The tumor suppressor gene p53 plays a central role in determining cell fate in response to DNA damage; cells may undergo either senescence or apoptosis, depending on cell type. Phosphorylation of Serine 46 (Ser⁴⁶) of p53 is considered to be a primary determinant for the induction of apoptosis, by selectively inducing transactivation of p53 target genes that have proapoptotic function. However, the generality of this mechanism of regulation of p53 remains a matter of debate. We investigated the role of p53 phosphorylation in adriamycin (ADR)-induced apoptosis. We found that Ser⁴⁶ was phosphorylated in four different cell lines undergoing ADR-induced senescence, as well as in two different cell lines undergoing ADR-induced apoptosis. Using alanine and glutamic acid substitution mutants of p53 Ser⁴⁶, we showed that Ser⁴⁶ phosphorylation is not a prerequisite for induction of the proapoptotic gene AIP1. These results indicate that Ser⁴⁶ phosphorylation of p53 is not required for ADR-induced apoptosis.

^* Correspondence: E-mail: sikawa{at}cir.tohoku.ac.jp