HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kunitomo, H. Articles by Iino, Y. Search for Related Content PubMed Articles by Kunitomo, H. Articles by Iino, Y.

¹ Molecular Genetics Research Laboratory, and
² Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo, Japan

Cilia and flagella play critical roles in cell motility, development and sensory perception in animals. Formation and maintenance of cilia require a conserved protein transport system called intraflagellar transport (IFT). Here, we show that Caenorhabditis elegans dyf-11 encodes an evolutionarily conserved protein required for cilium biogenesis. dyf-11 is expressed in most of the ciliated neurons and is regulated by DAF-19, a crucial transcription factor for ciliary genes in C. elegans. dyf-11 mutants exhibit stunted cilia, fluorescent dye-filling defects (Dyf) of sensory neurons, and abnormal chemotaxis (Che). Cell- and stage-specific rescue experiments indicated that DYF-11 is required for formation and maintenance of sensory cilia in cell-autonomous manner. Fluorescent protein-tagged DYF-11 localizes to cilia and moves antero- and retrogradely via IFT. Analysis of DYF-11 movement in bbs mutants further suggested that DYF-11 is likely associated with IFT complex B. Domain analysis using DYF-11 deletion constructs revealed that the coiled-coil region is required for proper localization and ciliogenesis. We further show that Traf3ip1/MIP-T3, the mammalian orthologue of DYF-11, localizes to cilia in the MDCK renal epithelial cells.

^* Correspondence: E-mail: iino{at}biochem.s.u-tokyo.ac.jp

This article has been cited by other articles:

				B. T. Emmer, D. Maric, and D. M. Engman Molecular mechanisms of protein and lipid targeting to ciliary membranes J. Cell Sci., February 15, 2010; 123(4): 529 - 536. [Abstract] [Full Text] [PDF]

				C. Iomini, L. Li, J. M. Esparza, and S. K. Dutcher Retrograde Intraflagellar Transport Mutants Identify Complex A Proteins With Multiple Genetic Interactions in Chlamydomonas reinhardtii Genetics, November 1, 2009; 183(3): 885 - 896. [Abstract] [Full Text] [PDF]