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¹ Graduate School of Biostudies, Kyoto University, Yoshida-konoe-cho, Kyoto 606-8501, Japan
² Graduate School of Science and Technology, Niigata University, Igarashi-2, Niigata 950-2181, Japan

The nuclear matrix has classically been assumed to be a solid structure coherently aligning nuclear components, but its real nature remains obscure. We separated the proteins in a ribonucleoprotein-containing nuclear matrix fraction of HeLa cells by reversed-phase HPLC followed by SDS-PAGE, and identified 83 proteins through peptide mass fingerprint (PMF) analysis. Many nucleolar proteins, classical nuclear matrix proteins, RNA binding proteins, cytoskeletal proteins and five uncharacterized proteins were identified in this fraction. Four of the latter proteins were localized to the cell nucleus, BXDC1 and EBNA1BP2 being especially localized to the nucleolus. Fluorescence recovery after photobleaching and RNAi knockdown analyses suggested that BXDC1 and EBNA1BP2 function in a dynamic scaffold for ribosome biogenesis.

^* Correspondence: hirano{at}lif.kyoto-u.ac.jp or thori{at}chem.sc.niigata-u.ac.jp