GTC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shimizu, A
Right arrow Articles by Miyazono, K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimizu, A
Right arrow Articles by Miyazono, K
GENES CELLS (1998) 3, 125-134.
Copyright © 1998 Blackwell Publishing or its licensors

Original Article

Identification of receptors and Smad proteins involved in activin signalling in a human epidermal keratinocyte cell line

A Shimizu, M Kato, A Nakao, T Imamura, P ten Dijke, CH Heldin, M Kawabata, S Shimada, and K Miyazono

BACKGROUND: Activin A is a multifunctional protein, which is a member of the transforming growth factor-beta (TGF-beta) superfamily. Smad proteins have recently been shown to transduce signals for the TGF-beta superfamily of proteins, and Smad2 was implicated in activin signalling in Xenopus embryos. RESULTS: We identified the receptors and Smad proteins activated by activin A in a human epidermal keratinocyte cell line, HaCaT. The major activin receptors expressed on HaCaT cells were activin type II receptor (ActR-II) and activin type IB receptor (ActR-IB). We have also shown that in HaCaT cells, activin A induced the phosphorylation of Smad3 and, to a lesser extent, of Smad2. On the other hand, TGF-beta induced an efficient phosphorylation of both Smad2 and Smad3. Activin A preferentially induced the nuclear translocation of Smad3 in HaCaT cells, whereas TGF-beta strongly induced the nuclear translocation of Smad2, as well as other Smads. Moreover, a constitutively active form of ActR-IB efficiently stimulated the formation of a heteromeric complex between Smad3 and Smad4 in COS cells transfected with Smad cDNAs. CONCLUSIONS: These results suggest that activin A binds to a receptor complex of ActR-II and ActR-IB, and preferentially activates Smad3 in HaCaT human keratinocytes.


This article has been cited by other articles:


Home page
 J BiochemHome page
S. Takano, F. Kanai, A. Jazag, H. Ijichi, J. Yao, H. Ogawa, N. Enomoto, M. Omata, and A. Nakao
Smad4 is Essential for Down-regulation of E-cadherin Induced by TGF-{beta} in Pancreatic Cancer Cell Line PANC-1
J. Biochem., March 1, 2007; 141(3): 345 - 351.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. Bamberger, A. Scharer, M. Antsiferova, B. Tychsen, S. Pankow, M. Muller, T. Rulicke, R. Paus, and S. Werner
Activin Controls Skin Morphogenesis and Wound Repair Predominantly via Stromal Cells and in a Concentration-Dependent Manner via Keratinocytes
Am. J. Pathol., September 1, 2005; 167(3): 733 - 747.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. WERNER and R. GROSE
Regulation of Wound Healing by Growth Factors and Cytokines
Physiol Rev, July 1, 2003; 83(3): 835 - 870.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
C. Welt, Y. Sidis, H. Keutmann, and A. Schneyer
Activins, Inhibins, and Follistatins: From Endocrinology to Signaling. A Paradigm for the New Millennium
Experimental Biology and Medicine, October 1, 2002; 227(9): 724 - 752.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Funaba and L. S. Mathews
Identification and Characterization of Constitutively Active Smad2 Mutants: Evaluation of Formation of Smad Complex and Subcellular Distribution
Mol. Endocrinol., October 1, 2000; 14(10): 1583 - 1591.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
X. Ni, S. Luo, T. Minegishi, and C. Peng
Activin A in JEG-3 Cells: Potential Role as an Autocrine Regulator of Steroidogenesis in Humans
Biol Reprod, May 1, 2000; 62(5): 1224 - 1230.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
W. Ishida, T. Hamamoto, K. Kusanagi, K. Yagi, M. Kawabata, K. Takehara, T. K. Sampath, M. Kato, and K. Miyazono
Smad6 Is a Smad1/5-induced Smad Inhibitor. CHARACTERIZATION OF BONE MORPHOGENETIC PROTEIN-RESPONSIVE ELEMENT IN THE MOUSE Smad6 PROMOTER
J. Biol. Chem., February 25, 2000; 275(9): 6075 - 6079.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
E. PIEK, C.-H. HELDIN, and P. TEN DIJKE
Specificity, diversity, and regulation in TGF-{beta} superfamily signaling
FASEB J, December 1, 1999; 13(15): 2105 - 2124.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
A. Franzén, E. Piek, B. Westermark, P. ten Dijke, and N.-E. Heldin
Expression of Transforming Growth Factor-{beta}1, Activin A, and Their Receptors in Thyroid Follicle Cells: Negative Regulation of Thyrocyte Growth and Function
Endocrinology, September 1, 1999; 140(9): 4300 - 4310.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
K. Yagi, D. Goto, T. Hamamoto, S. Takenoshita, M. Kato, and K. Miyazono
Alternatively Spliced Variant of Smad2 Lacking Exon 3. COMPARISON WITH WILD-TYPE Smad2 AND Smad3
J. Biol. Chem., January 8, 1999; 274(2): 703 - 709.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Chen, A. H. Borchers, Z. Dong, M. B. Powell, and G. T. Bowden
UVB Irradiation-induced Activator Protein-1 Activation Correlates with Increased c-fos Gene Expression in a Human Keratinocyte Cell Line
J. Biol. Chem., November 27, 1998; 273(48): 32176 - 32181.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
Copyright © 1998 by Wiley-Blackwell Publishing.