GTC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allcock, R.
Right arrow Articles by Price, P
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allcock, R.
Right arrow Articles by Price, P
GENES CELLS (2001) 6, 487-494.
Copyright © 2001 Blackwell Publishing or its licensors

Original Article

The central MHC gene, BAT1, may encode a protein that down-regulates cytokine production

RJ Allcock, JH Williams, and P Price

BACKGROUND: BAT1 belongs to the DEAD-box family of RNA-binding proteins and is encoded in the central MHC. To determine whether it affects immune responses and hence diseases influenced by MHC haplotypes, U937, THP1 and Jurkat cells were stably transfected with anti-sense DNA corresponding to exons 2-5 of BAT1 using a retroviral vector. RESULTS: Anti-sense transfectants carried anti-sense DNA and expressed anti-sense mRNA. After mitogenic stimulation, they produced higher levels of TNFalpha, IL-1 and IL-6 than equivalent cells carrying the vector alone, suggesting that BAT1 may down-regulate acute phase cytokine production. Polyclonal antibodies raised against a peptide in exon 8 of BAT1 recognized approximately 50 kDa and approximately 38 kDa proteins in all cell lines tested, including the anti-sense transfectants. Expression was localized to the nucleolus in dividing fibroblasts. However the immunochemistry may be confounded by a recently described gene, DDXL, on chromosome 19, which shares a 89% amino acid identity with BAT1. RT-PCR analyses established that BAT1 and DDXL mRNA are expressed in resting U937, THP1 and Jurkat cells. BAT1 and DDXL are divergent in the exons selected for the anti-sense study. CONCLUSIONS: BAT1 is a negative regulator of inflammation. Future studies should address how its functions relate to those of DDXL.


This article has been cited by other articles:


Home page
 Biol. Bull.Home page
S. V. Nyholm, J. Robidart, and P. R. Girguis
Coupling Metabolite Flux to Transcriptomics: Insights Into the Molecular Mechanisms Underlying Primary Productivity by the Hydrothermal Vent Tubeworm Ridgeia piscesae
Biol. Bull., June 1, 2008; 214(3): 255 - 265.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. P. Kota, S. R. Wagner, E. Huerta, J. M. Underwood, and J. A. Nickerson
Binding of ATP to UAP56 is necessary for mRNA export
J. Cell Sci., May 1, 2008; 121(9): 1526 - 1537.
[Abstract] [Full Text] [PDF]

Home page
 Arch Otolaryngol Head Neck SurgHome page
Z. Brownstein, A. Goldfarb, H. Levi, M. Frydman, and K. B. Avraham
Chromosomal mapping and phenotypic characterization of hereditary otosclerosis linked to the OTSC4 locus.
Arch Otolaryngol Head Neck Surg, April 1, 2006; 132(4): 416 - 424.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P. Price, A. M.-L. Wong, D. Williamson, D. Voon, S. Baltic, R. J.N. Allcock, A. Boodhoo, and F. T. Christiansen
Polymorphisms at positions -22 and -348 in the promoter of the BAT1 gene affect transcription and the binding of nuclear factors
Hum. Mol. Genet., May 1, 2004; 13(9): 967 - 974.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
Copyright © 2001 by Wiley-Blackwell Publishing.