GTC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pereira, P.
Right arrow Articles by Jones, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pereira, P.
Right arrow Articles by Jones, N.
GENES CELLS (2001) 6, 789-802.
Copyright © 2001 Blackwell Publishing or its licensors

Original Article

The RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating

PS Pereira and NC Jones

BACKGROUND: When nutritionally starved, the fission yeast Schizosaccharomyces pombe enters a cell differentiation process which leads to mating and meiosis. The Ste11 protein is a key regulator of this differentiation pathway, activating the transcription of mating and meiotic genes upon starvation. RESULTS: Here, we describe rgs1, a member of the Regulator of G-protein Signalling (RGS) family, as a novel Ste11 target gene. rgs1 expression requires both an Ste11-mediated nitrogen starvation signal and the pheromone-induced activation of the Byr2/Byr1/Spk1 MAPK pathway. We show that rgs1 deletion results in a sensitivity to pheromone and in a mating defect. Deltargs1 cells initiate the mating pathway normally, undergoing sexual agglutination and G1 arrest, while inducing pheromone-dependent transcription, but then fail to fuse with a mating partner while elongating abnormal conjugation tubes. Endogenous Rgs1 tagged with GFP localizes to the nucleus and cytoplasm, and this localization pattern is not altered during pheromone treatment. Importantly, Rgs1 function requires its C-terminal RGS domain, as well as a central DEP domain and a novel homology domain present in its N-terminal region (Fungal-DR domain). CONCLUSIONS: These results demonstrate that rgs1 expression requires nutritional starvation and pheromone signalling. Rgs1 negatively regulates pheromone signalling during mating, acting in a negative feedback loop that is essential for the mating process.


This article has been cited by other articles:


Home page
 Genes Dev.Home page
S. Kjaerulff, N. R. Andersen, M. T. Borup, and O. Nielsen
Cdk phosphorylation of the Ste11 transcription factor constrains differentiation-specific transcription to G1
Genes & Dev., February 1, 2007; 21(3): 347 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Mata and J. Bahler
Global roles of Ste11p, cell type, and pheromone in the control of gene expression during early sexual differentiation in fission yeast
PNAS, October 17, 2006; 103(42): 15517 - 15522.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
D. Dignard and M. Whiteway
SST2, a Regulator of G-Protein Signaling for the Candida albicans Mating Response Pathway
Eukaryot. Cell, January 1, 2006; 5(1): 192 - 202.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. Holmberg, O. Fleck, H. A. Hansen, C. Liu, R. Slaaby, A. M. Carr, and O. Nielsen
Ddb1 controls genome stability and meiosis in fission yeast
Genes & Dev., April 1, 2005; 19(7): 853 - 862.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. A. Roy, K. E. Lemberg, and P. Chidiac
Recruitment of RGS2 and RGS4 to the Plasma Membrane by G Proteins and Receptors Reflects Functional Interactions
Mol. Pharmacol., September 1, 2003; 64(3): 587 - 593.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
Copyright © 2001 by Wiley-Blackwell Publishing.