GTC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deguchi-Tawarada, M.
Right arrow Articles by Takai, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deguchi-Tawarada, M.
Right arrow Articles by Takai, Y.

CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CAST

Maki Deguchi-Tawarada1, Eiji Inoue1, Etsuko Takao-Rikitsu1, Marie Inoue1, Toshihisa Ohtsuka1,* and Yoshimi Takai2

1 KAN Research Institute, Kyoto Research Park, Shimogyo-ku, Kyoto 600-8815, Japan
2 Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Japan

The cytomatrix at the active zone (CAZ) is thought to define the site of Ca2+-dependent exocytosis of neurotransmitters. We have recently identified a novel CAZ protein from rat brain which we have named CAST (CAZ-associated structural protein). CAST forms a large molecular complex with other CAZ proteins such as Bassoon, RIM1 and Munc13-1, at least through direct binding to RIM1. Here, we have identified a rat protein that is structurally related to CAST and named it CAST2. Subcellular fractionation analysis of rat brain shows that CAST2 is also tightly associated with the postsynaptic density fraction. Like CAST, CAST2 directly binds RIM1 and forms a hetero-oligomer with CAST. In primary cultured rat hippocampal neurones, CAST2 co-localizes with Bassoon at synapses. Furthermore, immunoelectron microscopy reveals that CAST2 localizes to the vicinity of the presynaptic membrane of synapses in mouse brain. Sequence analysis reveals that CAST2 is a rat orthologue of the human protein ELKS. ELKS has also recently been identified as Rab6IP2 and ERC1. Accordingly, the original CAST is tentatively re-named CAST1. These results indicate that CAST2 is a new component of the CAZ and, together with CAST1, may be involved in the formation of the CAZ structure.

Communicated by: Shoichiro Tsukita

* Correspondence: Email: t-ohtsuka{at}kan.gr.jp




This article has been cited by other articles:


Home page
 Biophys. JHome page
M. Jose, D. K. Nair, W. D. Altrock, T. Dresbach, E. D. Gundelfinger, and W. Zuschratter
Investigating Interactions Mediated by the Presynaptic Protein Bassoon in Living Cells by Foerster's Resonance Energy Transfer and Fluorescence Lifetime Imaging Microscopy
Biophys. J., February 15, 2008; 94(4): 1483 - 1496.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. S. Andrews-Zwilling, H. Kawabe, K. Reim, F. Varoqueaux, and N. Brose
Binding to Rab3A-interacting Molecule RIM Regulates the Presynaptic Recruitment of Munc13-1 and ubMunc13-2
J. Biol. Chem., July 14, 2006; 281(28): 19720 - 19731.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
E. Inoue, M. Deguchi-Tawarada, E. Takao-Rikitsu, M. Inoue, I. Kitajima, T. Ohtsuka, and Y. Takai
ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells
Genes Cells, June 1, 2006; 11(6): 659 - 672.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Ko, C. Yoon, G. Piccoli, H. S. Chung, K. Kim, J.-R. Lee, H. W. Lee, H. Kim, C. Sala, and E. Kim
Organization of the Presynaptic Active Zone by ERC2/CAST1-Dependent Clustering of the Tandem PDZ Protein Syntenin-1
J. Neurosci., January 18, 2006; 26(3): 963 - 970.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
R. Hidajat, M. Nagano-Fujii, L. Deng, M. Tanaka, Y. Takigawa, S. Kitazawa, and H. Hotta
Hepatitis C virus NS3 protein interacts with ELKS-{delta} and ELKS-{alpha}, members of a novel protein family involved in intracellular transport and secretory pathways
J. Gen. Virol., August 1, 2005; 86(8): 2197 - 2208.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Ohara-Imaizumi, T. Ohtsuka, S. Matsushima, Y. Akimoto, C. Nishiwaki, Y. Nakamichi, T. Kikuta, S. Nagai, H. Kawakami, T. Watanabe, et al.
ELKS, a Protein Structurally Related to the Active Zone-associated Protein CAST, Is Expressed in Pancreatic {beta} Cells and Functions in Insulin Exocytosis: Interaction of ELKS with Exocytotic Machinery Analyzed by Total Internal Reflection Fluorescence Microscopy
Mol. Biol. Cell, July 1, 2005; 16(7): 3289 - 3300.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. L. Deken, R. Vincent, G. Hadwiger, Q. Liu, Z.-W. Wang, and M. L. Nonet
Redundant Localization Mechanisms of RIM and ELKS in Caenorhabditis elegans
J. Neurosci., June 22, 2005; 25(25): 5975 - 5983.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
Copyright © 2004 by Wiley-Blackwell Publishing.