GTC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Genes to Cells (2004) 9, 1055-1067. doi:10.1111/j.1365-2443.2004.00789.x
© 2004 Blackwell Publishing or its licensors
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamamoto, M.
Right arrow Articles by Taniguchi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, M.
Right arrow Articles by Taniguchi, S.

ASC is essential for LPS-induced activation of procaspase-1 independently of TLR-associated signal adaptor molecules

Masatatsu Yamamoto1,2, Katsuyuki Yaginuma2, Hiroko Tsutsui3,4, Junji Sagara1, Xin Guan1, Ekihiro Seki3,4, Koubun Yasuda3,4, Masahiro Yamamoto5, Shizuo Akira5, Kenji Nakanishi3,4, Tetsuo Noda2,6,7,a and Shun’ichiro Taniguchi1,*

1 Department of Molecular Oncology Division of Molecular and Cellular Biology Institute on Ageing and Adaptation, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan
2 Department of Cell Biology, Cancer Research Institute of the Japanese Foundation of Cancer Research, 1-37-1 Kamiikebukuro, Toshima-ku, Tokyo 170-8455, Japan
3 Department of Immunology and Medical Zoology, Hyogo College of Medicine, 1-1 Mukogawa, Nishinomiya 663-8501, Japan
4 Core Research of Evolutional Science and Technology, Japan Science and Technology agency, 4-1-8 Motomati, Kawaguchi 332-0012, Japan
5 Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, 3-1, Yamada-oka, Suita, Osaka 565-0871, Japan
6 Department of Molecular Genetics, Tohoku University School of Medicine, 2-1 Seiryo-cho, Aoba-Ku, Sendai 980-8575, Japan
7 Mouse Functional Genomics Research Group, Institute of Physical and Chemical Research (Japan) (RIKEN) Genomic Sciences Center, 214 Maeda-cho, Totsuka-ku, Yokohama, Kanagawa 244-0804, Japan

Toll-like receptors (TLRs) initiate a signalling cascade via association with an adaptor molecule, myeloid differentiation factor 88 (MyD88) and/or TIR domain-containing adaptor inducing-IFN-ß (Trif), to induce various pro-inflammatory cytokines for microbial eradication. After stimulation of TLR4 with lipopolysaccharide (LPS), both IL-1ß and IL-18 are processed, depending on the activation of caspase-1, although its mechanism remains unclear. ASC is an adapter protein possibly involved in the activation of procaspase-1. To unravel the requirement of ASC, we generated Asc–/– mice. Upon stimulation with LPS, Asc–/– macrophages failed in the processing of procaspase-1 and maturation of pro-IL-1ß and pro-IL-18, but normally produced other pro-inflammatory cytokines including TNF-{alpha} and IL-6. MyD88–/– and Trif–/– macrophages showed normal activation of caspase-1, demonstrating a dispensable role for MyD88 and Trif. After, LPS-challenged Asc–/– mice lacked serum elevation of IL-1ß and IL-18. Moreover, the Asc–/– mice exhibited neither acute liver injury nor lethal shock. These results demonstrate critical roles for ASC in the release of IL-1ß/IL-18 via activation of caspase-1 and provide new insights into the inflammatory responses for host defence and diseases.

Communicated by: Shinichi Aizawa

a Requests for mice: E-mail: tnoda{at}ims.u-tokyo.ac.jp

* Correspondence: E-mail: stangch{at}sch.md.shinshu-u.ac.jp




This article has been cited by other articles:


Home page
 IOVSHome page
D. Zoukhri, S. Ko, P. C. Stark, and C. L. Kublin
Roles of Caspase 1 and Extracellular Signal-Regulated Kinase in Inflammation-Induced Inhibition of Lacrimal Gland Protein Secretion
Invest. Ophthalmol. Vis. Sci., October 1, 2008; 49(10): 4392 - 4398.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
J. Maelfait, E. Vercammen, S. Janssens, P. Schotte, M. Haegman, S. Magez, and R. Beyaert
Stimulation of Toll-like receptor 3 and 4 induces interleukin-1{beta} maturation by caspase-8
J. Exp. Med., September 1, 2008; 205(9): 1967 - 1973.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
N. Yajima, M. Takahashi, H. Morimoto, Y. Shiba, Y. Takahashi, J. Masumoto, H. Ise, J. Sagara, J. Nakayama, S. Taniguchi, et al.
Critical Role of Bone Marrow Apoptosis-Associated Speck-Like Protein, an Inflammasome Adaptor Molecule, in Neointimal Formation After Vascular Injury in Mice
Circulation, June 17, 2008; 117(24): 3079 - 3087.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Stehlik and A. Dorfleutner
COPs and POPs: Modulators of Inflammasome Activity
J. Immunol., December 15, 2007; 179(12): 7993 - 7998.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
M. Lamkanfi, T.-D. Kanneganti, L. Franchi, and G. Nunez
Caspase-1 inflammasomes in infection and inflammation
J. Leukoc. Biol., August 1, 2007; 82(2): 220 - 225.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Seshadri, M. D. Duncan, J. M. Hart, M. A. Gavrilin, and M. D. Wewers
Pyrin Levels in Human Monocytes and Monocyte-Derived Macrophages Regulate IL-1beta Processing and Release
J. Immunol., July 15, 2007; 179(2): 1274 - 1281.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Franchi, T.-D. Kanneganti, G. R. Dubyak, and G. Nunez
Differential Requirement of P2X7 Receptor and Intracellular K+ for Caspase-1 Activation Induced by Intracellular and Extracellular Bacteria
J. Biol. Chem., June 29, 2007; 282(26): 18810 - 18818.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. A. Kummer, R. Broekhuizen, H. Everett, L. Agostini, L. Kuijk, F. Martinon, R. van Bruggen, and J. Tschopp
Inflammasome Components NALP 1 and 3 Show Distinct but Separate Expression Profiles in Human Tissues Suggesting a Site-specific Role in the Inflammatory Response
J. Histochem. Cytochem., May 1, 2007; 55(5): 443 - 452.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. W. Moore, A. R. Cruz, C. J. LaVake, A. L. Marzo, C. H. Eggers, J. C. Salazar, and J. D. Radolf
Phagocytosis of Borrelia burgdorferi and Treponema pallidum Potentiates Innate Immune Activation and Induces Gamma Interferon Production
Infect. Immun., April 1, 2007; 75(4): 2046 - 2062.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. Dorfleutner, N. B. Bryan, S. J. Talbott, K. N. Funya, S. L. Rellick, J. C. Reed, X. Shi, Y. Rojanasakul, D. C. Flynn, and C. Stehlik
Cellular Pyrin Domain-Only Protein 2 Is a Candidate Regulator of Inflammasome Activation
Infect. Immun., March 1, 2007; 75(3): 1484 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. M. Miggin, E. Palsson-McDermott, A. Dunne, C. Jefferies, E. Pinteaux, K. Banahan, C. Murphy, P. Moynagh, M. Yamamoto, S. Akira, et al.
NF-{kappa}B activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1
PNAS, February 27, 2007; 104(9): 3372 - 3377.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. M. Cruz, A. Rinna, H. J. Forman, A. L. M. Ventura, P. M. Persechini, and D. M. Ojcius
ATP Activates a Reactive Oxygen Species-dependent Oxidative Stress Response and Secretion of Proinflammatory Cytokines in Macrophages
J. Biol. Chem., February 2, 2007; 282(5): 2871 - 2879.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. J. Taxman, J. Zhang, C. Champagne, D. T. Bergstralh, H. A. Iocca, J. D. Lich, and J. P.-Y. Ting
Cutting Edge: ASC Mediates the Induction of Multiple Cytokines by Porphyromonas gingivalis via Caspase-1-Dependent and -Independent Pathways
J. Immunol., October 1, 2006; 177(7): 4252 - 4256.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. Franchi, C. McDonald, T.-D. Kanneganti, A. Amer, and G. Nunez
Nucleotide-Binding Oligomerization Domain-Like Receptors: Intracellular Pattern Recognition Molecules for Pathogen Detection and Host Defense
J. Immunol., September 15, 2006; 177(6): 3507 - 3513.
[Abstract] [Full Text] [PDF]

Home page
 Innate ImmunityHome page
F. S. Sutterwala, Y. Ogura, D. S. Zamboni, C. R. Roy, and R. A. Flavell
NALP3: a key player in caspase-1 activation
Innate Immunity, August 1, 2006; 12(4): 251 - 256.
[Abstract] [PDF]

Home page
 JEMHome page
M. Lara-Tejero, F. S. Sutterwala, Y. Ogura, E. P. Grant, J. Bertin, A. J. Coyle, R. A. Flavell, and J. E. Galan
Role of the caspase-1 inflammasome in Salmonella typhimurium pathogenesis
J. Exp. Med., June 12, 2006; 203(6): 1407 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Ozoren, J. Masumoto, L. Franchi, T.-D. Kanneganti, M. Body-Malapel, I. Erturk, R. Jagirdar, L. Zhu, N. Inohara, J. Bertin, et al.
Distinct Roles of TLR2 and the Adaptor ASC in IL-1beta/IL-18 Secretion in Response to Listeria monocytogenes
J. Immunol., April 1, 2006; 176(7): 4337 - 4342.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. M. Kahlenberg, K. C. Lundberg, S. B. Kertesy, Y. Qu, and G. R. Dubyak
Potentiation of Caspase-1 Activation by the P2X7 Receptor Is Dependent on TLR Signals and Requires NF-{kappa}B-Driven Protein Synthesis
J. Immunol., December 1, 2005; 175(11): 7611 - 7622.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Noulin, V. F. J. Quesniaux, S. Schnyder-Candrian, B. Schnyder, I. Maillet, T. Robert, B. B. Vargaftig, B. Ryffel, and I. Couillin
Both Hemopoietic and Resident Cells Are Required for MyD88-Dependent Pulmonary Inflammatory Response to Inhaled Endotoxin
J. Immunol., November 15, 2005; 175(10): 6861 - 6869.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
Copyright © 2004 by Wiley-Blackwell Publishing.