| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Biology, School of Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
2 Graduate School of Biological Science, Nara Institute of Science and Technology, Takayama, Ikoma, Nara 630-0101 Japan
3 Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-0814, Japan
4 Department of Ophthalmology, Nagoya University, School of Medicine, 65 Tsurumai, Naka-ku, Nagoya 466-8550, Japan
The sister chromatid cohesion factor Chl12 shares amino acid sequence similarity with RFC1, the largest subunit of replication factor C (RFC), and forms a clamp loader complex in association with the RFC small subunits RFCs2-5. It has been shown that the human Chl12-RFC complex, reconstituted with a baculovirus expression system, specifically interacts with human proliferating cell nuclear antigen (PCNA). The purified Chl12-RFC complex is structurally indistinguishable from RFC, as shown by electron microscopy, and it exhibits DNA-stimulated ATPase activity that is further enhanced by PCNA, and by DNA binding activity on specific primer/template DNA structures. Furthermore, the complex loads PCNA onto a circular DNA substrate, and stimulates DNA polymerase 
DNA synthesis on a primed M13 single-stranded template in the presence of purified replication proteins. However, it cannot substitute for RFC in promoting simian virus 40 DNA replication in vitro with crude fractions. These results demonstrate that the human Chl12-RFC complex is a second PCNA loader and that its roles in replication are clearly distinguishable from those of RFC.
Present address: aAmersham Biosciences K.K. Sanken Bldg. 3-25-1 Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan; bDepartment of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan * Correspondence: E-mail: ttsurscb{at}mbox.nc.kyushu-u.ac.jp
This article has been cited by other articles:
|
|
 |
|
  A. Farina, J.-H. Shin, D.-H. Kim, V. P. Bermudez, Z. Kelman, Y.-S. Seo, and J. Hurwitz Studies with the Human Cohesin Establishment Factor, ChlR1: ASSOCIATION OF ChlR1 WITH Ctf18-RFC AND Fen1 J. Biol. Chem., July 25, 2008; 283(30): 20925 - 20936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Berkowitz, K. H. Kaestner, and T. A. Jongens Germline expression of mammalian CTF18, an evolutionarily conserved protein required for germ cell proliferation in the fly and sister chromatid cohesion in yeast Mol. Hum. Reprod., March 1, 2008; 14(3): 143 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. B. Ansbach, C. Noguchi, I. W. Klansek, M. Heidlebaugh, T. M. Nakamura, and E. Noguchi RFCCtf18 and the Swi1-Swi3 Complex Function in Separate and Redundant Pathways Required for the Stabilization of Replication Forks to Facilitate Sister Chromatid Cohesion in Schizosaccharomyces pombe Mol. Biol. Cell, February 1, 2008; 19(2): 595 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shiomi, C. Masutani, F. Hanaoka, H. Kimura, and T. Tsurimoto A Second Proliferating Cell Nuclear Antigen Loader Complex, Ctf18-Replication Factor C, Stimulates DNA Polymerase {eta} Activity J. Biol. Chem., July 20, 2007; 282(29): 20906 - 20914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xu, C. Boone, and G. W. Brown Genetic Dissection of Parallel Sister-Chromatid Cohesion Pathways Genetics, July 1, 2007; 176(3): 1417 - 1429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sabbioneda, B. K. Minesinger, M. Giannattasio, P. Plevani, M. Muzi-Falconi, and S. Jinks-Robertson The 9-1-1 Checkpoint Clamp Physically Interacts with Pol{zeta} and Is Partially Required for Spontaneous Pol{zeta}-dependent Mutagenesis in Saccharomyces cerevisiae J. Biol. Chem., November 18, 2005; 280(46): 38657 - 38665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kim, K. Robertson, K. J. L. Mylonas, F. C. Gray, I. Charapitsa, and S. A. MacNeill Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast Nucleic Acids Res., July 21, 2005; 33(13): 4078 - 4089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. O. Bylund and P. M. J. Burgers Replication Protein A-Directed Unloading of PCNA by the Ctf18 Cohesion Establishment Complex Mol. Cell. Biol., July 1, 2005; 25(13): 5445 - 5455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tsurimoto, A. Shinozaki, M. Yano, M. Seki, and T. Enomoto Human Werner helicase interacting protein 1 (WRNIP1) functions as a novel modulator for DNA polymerase {delta} Genes Cells, January 1, 2005; 10(1): 13 - 22. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
