| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Molecular Pathology and 2 Center for the Development of Molecular Target Drugs, Cancer Research Institute, Kanazawa University; Ishikawa 920-0934, Japan
3 Department of Radiology, Graduate School of Medicine, Kanazawa University, Kanazawa, Ishikawa 920-0934, Japan
The Rad17-replication factor C (Rad17-RFC) and Rad9-Rad1-Hus1 complexes are thought to function in the early phase of cell-cycle checkpoint control as sensors for genome damage and genome replication errors. However, genetic analysis of the functions of these complexes in vertebrates is complicated by the lethality of these gene disruptions in embryonic mouse cells. We disrupted the Rad17 and Rad9 loci by gene targeting in the chicken B lymphocyte line DT40. Rad17–/– and Rad9–/– DT40 cells are viable, and are highly sensitive to UV irradiation, alkylating agents, and DNA replication inhibitors, such as hydroxyurea. We further found that Rad17–/– and Rad9–/– but not ATM–/– cells are defective in S-phase DNA damage checkpoint controls and in the cellular response to stalled DNA replication. These results indicate a critical role for chicken Rad17 and Rad9 in the cellular response to stalled DNA replication and DNA damage.
*Correspondence: E-mail: kyamamot{at}kenroku.kanazawa-u.ac.jp
This article has been cited by other articles:
|
|
 |
|
  A. Saberi, M. Nakahara, J. E. Sale, K. Kikuchi, H. Arakawa, J.-M. Buerstedde, K. Yamamoto, S. Takeda, and E. Sonoda The 9-1-1 DNA Clamp Is Required for Immunoglobulin Gene Conversion Mol. Cell. Biol., October 1, 2008; 28(19): 6113 - 6122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Hu, Y. Liu, C. Zhang, Y. Zhao, W. He, L. Han, L. Yang, K. M. Hopkins, X. Yang, H. B. Lieberman, et al. Targeted Deletion of Rad9 in Mouse Skin Keratinocytes Enhances Genotoxin-Induced Tumor Development Cancer Res., July 15, 2008; 68(14): 5552 - 5561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Delacroix, J. M. Wagner, M. Kobayashi, K.-i. Yamamoto, and L. M. Karnitz The Rad9-Hus1-Rad1 (9-1-1) clamp activates checkpoint signaling via TopBP1 Genes & Dev., June 15, 2007; 21(12): 1472 - 1477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yong, S. Bao, H. Chen, D. Li, E. R. Sanchez, and W. Shou Mice Lacking Protein Phosphatase 5 Are Defective in Ataxia Telangiectasia Mutated (ATM)-mediated Cell Cycle Arrest J. Biol. Chem., May 18, 2007; 282(20): 14690 - 14694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Boerckel, D. Walker, and S. Ahmed The Caenorhabditis elegans Rad17 Homolog HPR-17 Is Required for Telomere Replication Genetics, May 1, 2007; 176(1): 703 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Harris, M. Lowden, I. Clejan, M. Tzoneva, J. H. Thomas, J. Hodgkin, and S. Ahmed Mutator Phenotype of Caenorhabditis elegans DNA Damage Checkpoint Mutants Genetics, October 1, 2006; 174(2): 601 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kobayashi, H. Ono, K. Mihara, H. Tauchi, K. Komatsu, T. Shibata, H. Shimizu, K. Uchida, and K.-i. Yamamoto ATM activation by a sulfhydryl-reactive inflammatory cyclopentenone prostaglandin. Genes Cells, July 1, 2006; 11(7): 779 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Shiotani, M. Kobayashi, M. Watanabe, K.-i. Yamamoto, T. Sugimura, and K. Wakabayashi Involvement of the ATR- and ATM-Dependent Checkpoint Responses in Cell Cycle Arrest Evoked by Pierisin-1 Mol. Cancer Res., February 1, 2006; 4(2): 125 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Dang, S. Bao, and X.-F. Wang Human Rad9 is required for the activation of S-phase checkpoint and the maintenance of chromosomal stability Genes Cells, April 1, 2005; 10(4): 287 - 295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kumar and J. A. Huberman On the Slowing of S Phase in Response to DNA Damage in Fission Yeast J. Biol. Chem., October 15, 2004; 279(42): 43574 - 43580. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | ADVANCED SEARCH | TABLE OF CONTENTS |
