GTC
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Genes to Cells (2004) 9, 419-428. doi:10.1111/j.1356-9597.2004.00737.x
© 2004 Blackwell Publishing or its licensors
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishida, M.
Right arrow Articles by Ohta, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishida, M.
Right arrow Articles by Ohta, T.

Transcriptional co-activator activity of SYT is negatively regulated by BRM and Brg1

Michiko Ishida1, Shinya Tanaka2, Misao Ohki1 and Tsutomu Ohta1,*

1 Center for Medical Genomics, National Cancer Center Research Institute, 5-1-1 Tsukiji Chuo-ku, Tokyo 104-0045, Japan
2 Laboratory of Molecular and Cellular Pathology, Hokkaido University School of Medicine, N15, W7, Kita-ku, Sapporo 060-8638, Japan

The t(X;18)(p11.2;q11.2) translocation found in synovial sarcomas results in the fusion of the SYT gene on chromosome 18 to the SSX gene on chromosome X. Although the SYT-SSX fusion proteins may trigger synovial sarcoma development, the biological functions of SYT, SSX and SYT-SSX genes are unclear. Transfections of Gal4 DNA binding domain fusion protein constructs demonstrate that SYT protein acts as a transcriptional co-activator at the C-terminal domain and that the activity is repressed through the N-terminus. The N-terminal 70 amino acids of SYT bind not only to BRM, but also to Brg1, both of which are subunits of SWI/SNF chromatin remodelling complexes. Here, we have investigated the functions of BRM and Brg1 on the repression of SYT activity. The negative regulation of SYT transcriptional co-activator activity is dependent on the ATP-hydrolysis of BRM and Brg1 in the protein complexes. This indicates that the SWI/SNF protein complexes regulate SYT activity using the chromatin remodelling activity.

Communicated by: Masayuki M. Yamamoto

* Correspondence: Email: cota{at}gan2.res.ncc.go.jp




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
M. Ishida, M. Miyamoto, S. Naitoh, D. Tatsuda, T. Hasegawa, T. Nemoto, H. Yokozeki, K. Nishioka, A. Matsukage, M. Ohki, et al.
The SYT-SSX Fusion Protein Down-Regulates the Cell Proliferation Regulator COM1 in t(x;18) Synovial Sarcoma
Mol. Cell. Biol., February 15, 2007; 27(4): 1348 - 1355.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Zhang, T. Ohta, A. Maruyama, T. Hosoya, K. Nishikawa, J. M. Maher, S. Shibahara, K. Itoh, and M. Yamamoto
BRG1 Interacts with Nrf2 To Selectively Mediate HO-1 Induction in Response to Oxidative Stress
Mol. Cell. Biol., November 1, 2006; 26(21): 7942 - 7952.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
Y. Inayoshi, H. Kaneoka, Y. Machida, M. Terajima, T. Dohda, K. Miyake, and S. Iijima
Repression of GR-Mediated Expression of the Tryptophan Oxygenase Gene by the SWI/SNF Complex during Liver Development
J. Biochem., October 1, 2005; 138(4): 457 - 465.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Iwasaki, N. Koibuchi, and W. W. Chin
Synovial Sarcoma Translocation (SYT) Encodes a Nuclear Receptor Coactivator
Endocrinology, September 1, 2005; 146(9): 3892 - 3899.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE ADVANCED SEARCH TABLE OF CONTENTS
Copyright © 2004 by Wiley-Blackwell Publishing.